Noonan 3d

General

Cell Line

hPSCreg name MRIi026-A
Cite as:
MRIi026-A (RRID:CVCL_A0SX)
Alternative name(s)
Noonan 3d
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
MRIi025-A
(Noonan 3a)
Donor diseases:
Noonan Syndrome
KSCBi010-A
(DKH005i-A, DKHi005-A)
Donor diseases:
Senior-Loken syndrome
KMUGMCi003-A
(KMUGMCi003ERCC2, KN610)
Donor diseases:
Trichothiodystrophy 1, Photosensitive
KMUGMCi002-A
(KMUGMCi002NIPBL)
Donor diseases:
Cornelia De Lange Syndrome
PCIi026-A
(PC085)
Donor diseases:
Waardenburg syndrome
PFIZi032-A
(B214c8)
Donor's gene variants:
TSC2
Donor diseases:
Tuberous Sclerosis
PCIi025-A
(PC084)
Donor diseases:
Waardenburg syndrome
PCIi032-A
(PC087)
Donor diseases:
Waardenburg Syndrome
IMBAi017-A
(B002-ARID1B#8)
Donor diseases:
Coffin-Siris Syndrome
UOMi010-A
(ANCS-hiPSC-HPP5)
Donor diseases:
Hypophosphatasia
IMAGINi022-A
(IMAGINE022)
Donor diseases:
Waardenburg syndrome
UOMi011-A
(ANCS-hiPSC-HPP6)
Donor diseases:
Hypophosphatasia
Last update 20th April 2022
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Provider

Generator Klinikum rechts der Isar (MRI)

External Databases

BioSamples SAMEA9468451
Cellosaurus CVCL_A0SX
Wikidata Q108820974

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female
Ethnicity caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • Noonan's Syndrome
  • Noonan syndrome
  • Noonan Syndrome

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

External Databases (Donor)

BioSamples SAMEA9468452

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ehtikkommission, Fakultät für Medizin, Klinikum rechts der Isar (TUM)
Approval number 2109/08
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC
Source cell origin
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
  • Reticuloendothelial System, Blood
  • Peripheral Blood
  • peripheral blood
  • blood
  • Blood
  • Whole Blood
  • BLOOD
  • portion of blood
  • vertebrate blood
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Dispase
CO2 Concentration 5 %
Medium Essential 8™
Supplements
E8 supplement 10 ml
Penicillin/Streptomycin 2,5 ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-81
Yes
NANOG
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
SOX7 SRY-box transcription factor 7
Unknown
Protocol or reference
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Protocol or reference
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
KRT14 keratin 14
Unknown
Protocol or reference

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Unknown

Other Genotyping (Cell Line)