Generation and characterization of human iPSC lines SANi001-A and SANi002-A from mobilized peripheral blood derived megakaryoblasts
Summary
Mobilized peripheral blood (MPB) CD34+ cells were differentiated to CD34+/CD41+ megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population which was reprogrammed with a hOKSM self-silencing polycistronic lentiviral vector. Resulting iPSC showed normal karyotype and expression of pluripotency associated markers and in vitro spontaneous differentiation towards the 3 germ layers confirmed pluripotency of iPSC lines. Besides normal iPSC applications, these lines can be used as a control line for other megakaryoid origin iPSC and could be applied for epigenetic based research. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Hansen M, Varga E, Wüst T, Mellink C, van der Kevie-Kersemaekers AM, von Lindern M, van den Akker E |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2017 Dec;25:42-45 |
| PubMed | 29055227 |
| DOI | 10.1016/j.scr.2017.10.002 |