C27, iPS-27

General

Cell Line

hPSCreg name MSKi002-A
Cite as:
MSKi002-A (RRID:CVCL_D0JC)
Alternative name(s)
C27, iPS-27
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 9th August 2023
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Provider

Generator Memorial Sloan Kettering Cancer Center (MSK)

External Databases

BioSamples SAMEA114243953
Cellosaurus CVCL_D0JC
Wikidata Q123032724

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:

Donor cells were from ATCC (MRC-5; CCL-171).

Donor Information

General Donor Information

Sex male
Ethnicity White, 14 weeks gestation

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA114243995

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? No
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. Donor cell was purchased from ATCC (MRC-5 CCL-17). Please contact ATCC at www.atcc.org.
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information Please contact ATCC at www.atcc.org.
Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? Yes
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell line name MRC5
Source cell type
Homo sapiens (human) lung; fibroblast; normal cell line. The MRC-5 cell line was derived from normal lung tissue of a 14-week-old male fetus by J.P. Jacobs in September of 1966.
Synonyms
  • MRC-5
  • MRC-5 cell
  • MRC-V cell
  • MRC5 cell
  • MRC-V
  • MRCV
  • Medical Research Council cell strain-5
show more synonyms
Source cell line vendor ATCC

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
No
Reprogramming vectors silenced?
Unknown
Notes on reprogramming vector silencing Pronounced lentiviral vector silencing was a characteristic of successfully reprogrammed hiPSC clones, but lack of complete silencing.

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones Gene Expression Profiling; Teratoma Formation; In Vitro Differentiation; Vector silencing.
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Non coated
Feeder cells on a feeder layer of mitomycin C-treated mouse embryonic fibroblasts (MEFs)
Passage method Enzymatically
Dispase
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM/F12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
NEAA 1 %
Glutamax 1 %
2-mercaptoethanol 0.1 %
bFGF 6 ng/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-3
Yes
NANOG
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Morphology
From Left to Right: neuroectoderm(black arrow),smooth muscle(blank arrow),and primitive myxoid tissue (arrowhead), 4x. Pigmented epithelial tissue compatible with retinal neuroectoderm, 40x. Intestinal like epithelium including goblet cells (endoderm), 40x. Smooth muscle tissue, 20x. Inset demonstrates a high power image of immature mesenchymal tissue, potentially cartilage (40x).
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal male
Passage number: 12

Other Genotyping (Cell Line)