Generation of a PXR reporter human induced pluripotent stem cell line (PXR-mCherry hiPSC) using the CRISPR/Cas9 system
Summary
Pregnane X receptor (PXR) is a key nuclear receptor that mediates drug metabolism and stimulates hepatocyte proliferation. However, the lack of PXR expression in human pluripotent stem cell-derived hepatocytes limits their application for drug screening and toxicity testing. Here, we generated a PXR-mCherry reporter human induced pluripotent stem cell (hiPSC) line using the CRISPR/Cas9 system. PXR-mCherry hiPSCs were pluripotent and had differentiation potential and a normal karyotype. This cell line is an important tool for identifying factors that increase PXR-mediated drug metabolism and hepatocyte proliferation. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Kim H, Kim JW, Kim SJ, Choi YJ, Kim DS, Park HJ |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2018 Jan;26:72-75 |
| PubMed | 29247817 |
| DOI | 10.1016/j.scr.2017.12.001 |