C25

General

Cell Line

hPSCreg name TUMi001-A
Cite as:
TUMi001-A (RRID:CVCL_VF08)
Alternative name(s)
C25
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
MRIi001-A-1
(C6-AAVS1-iCasRx)
Last update 20th January 2021
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Provider

Generator Technische Universität München (TUM)
Owner Technische Universität München (TUM)
Derivation country Germany

External Databases

BioSamples SAMEA104132875
Cellosaurus CVCL_VF08
Wikidata Q54973375

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104132876

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Unknown
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethikkommission der Fakultät für Medizin der Technischen Universität München
Approval number 2109/08
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Ethikkommission der Fakultät für Medizin der Technischen Universität München
Approval number 2109/08
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Any skin fibroblast that is part of some dermis.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
Yes
Absence of reprogramming vector(s)?
No
Reprogramming vectors silenced?
Yes
Methods used
Immunostaining, PCR, RT-PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium Other medium:
Base medium: DMEM/F12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-glutamine 2 mM
nonessential amino acids 0,1 mM
beta-mercaptoethanol 0,1 mM
penicillin 50 U/ml
streptomycin 50 µg/ml
human b-FGF 10 ng/ml

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
TRA 1-81
Yes
SOX2
Yes
c-MYC
Yes
KLF4
Yes
POU5F1 (OCT-4)
Yes
REX1
Yes
TDGF1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
PDX1
Yes
SOX7
Yes
AFP
Yes
Protocol or reference
Moretti et al_2010_New Engl J Med_Patient-specific induced pluripotent stem-cell models for long-QT syndrome_appendixSupFig2.pdf
Up-regulation of lineage markers representative of the three embryonic germ layers in induced pluripotent stem cell(iPSC) clones from control and LQT1 individuals (C25 iPSC come from 1 of the 2 Control individuals).
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
PECAM1 (CD31)
Yes
DES
Yes
ACTA2
Yes
MYL2
Yes
CDH5
Yes
SCL
Yes
Protocol or reference
Moretti et al_2010_New Engl J Med_Patient-specific induced pluripotent stem-cell models for long-QT syndrome_appendixSupFig2.pdf
Up-regulation of lineage markers representative of the three embryonic germ layers in induced pluripotent stem cell(iPSC) clones from control and LQT1 individuals (C25 iPSC come from 1 of the 2 Control individuals).
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
KRT14
Yes
NCAM1
Yes
TH
Yes
GABRR2
Yes
Protocol or reference
Moretti et al_2010_New Engl J Med_Patient-specific induced pluripotent stem-cell models for long-QT syndrome_appendixSupFig2.pdf
Up-regulation of lineage markers representative of the three embryonic germ layers in induced pluripotent stem cell(iPSC) clones from control and LQT1 individuals (C25 iPSC come from 1 of the 2 Control individuals).

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Unknown

Other Genotyping (Cell Line)