C25

General#

Cell Line

hPSCreg Name TUMi001-A
Alternative name(s)
C25
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 16th January 2018
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Provider

Generator Technische Universität München (TUM)
Owner Technische Universität München (TUM)
Derivation country Germany

External Databases

BioSamples SAMEA104132875
Cellosaurus CVCL_VF08

General Information

Publications
Projects
Is the cell line available in principle?
No

Donor Information#

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA104132876

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
Yes
Absence of reprogramming vector(s)?
No
Reprogramming vectors silenced?
Yes
Methods used
Immunostaining, PCR, RT-PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Medium Other medium:
Base medium: DMEM/F12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-glutamine 2 mM
nonessential amino acids 0,1 mM
beta-mercaptoethanol 0,1 mM
penicillin 50 U/ml
streptomycin 50 µg/ml
human b-FGF 10 ng/ml

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
TRA 1-81
Yes
SOX2
Yes
c-MYC
Yes
KLF4
Yes
OCT3/4
Yes
REX1
Yes
TDGF1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
PDX1
Yes
SOX7
Yes
AFP
Yes
Protocol or reference
Moretti et al_2010_New Engl J Med_Patient-specific induced pluripotent stem-cell models for long-QT syndrome_appendixSupFig2.pdf
Up-regulation of lineage markers representative of the three embryonic germ layers in induced pluripotent stem cell(iPSC) clones from control and LQT1 individuals (C25 iPSC come from 1 of the 2 Control individuals).
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
CD31
Yes
DES
Yes
ACTA2
Yes
MYL2
Yes
CDH5
Yes
SCL
Yes
Protocol or reference
Moretti et al_2010_New Engl J Med_Patient-specific induced pluripotent stem-cell models for long-QT syndrome_appendixSupFig2.pdf
Up-regulation of lineage markers representative of the three embryonic germ layers in induced pluripotent stem cell(iPSC) clones from control and LQT1 individuals (C25 iPSC come from 1 of the 2 Control individuals).
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
KRT14
Yes
NCAM1
Yes
TH
Yes
GABRR2
Yes
Protocol or reference
Moretti et al_2010_New Engl J Med_Patient-specific induced pluripotent stem-cell models for long-QT syndrome_appendixSupFig2.pdf
Up-regulation of lineage markers representative of the three embryonic germ layers in induced pluripotent stem cell(iPSC) clones from control and LQT1 individuals (C25 iPSC come from 1 of the 2 Control individuals).

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Unkown

Other Genotyping (Cell Line)