C25

General#

Cell Line

hPSCreg Name TUMi001-A
Alternative name(s)
C25
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 22nd May 2019
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Technische Universität München (TUM)
Owner Technische Universität München (TUM)
Derivation country Germany

External Databases

BioSamples SAMEA104132875
Cellosaurus CVCL_VF08

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA104132876

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Provide contact information of the holder of the original Donor Information Sheet:
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? No
If you do not hold the SIGNED Donor Consent Form, do you know who does? No
Contact information / weblink
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? Yes
Alternatives to consent
Alternative consent approval number
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Unknown
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent pertain to a specific research project? No
Details on restriction to research project
Does consent permit unforeseen future research, without further consent? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Policy for access to genetic information No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Please describe how access is provided:
Contact data, institution, or website:
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethikkommission der Fakultät für Medizin der Technischen Universität München
Approval number 2109/08
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Ethikkommission der Fakultät für Medizin der Technischen Universität München
Approval number 2109/08
Please describe:
Further constraints on use
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Constraints for use or distribution

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
Yes
Absence of reprogramming vector(s)?
No
Reprogramming vectors silenced?
Yes
Methods used
Immunostaining, PCR, RT-PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Medium Other medium:
Base medium: DMEM/F12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-glutamine 2 mM
nonessential amino acids 0,1 mM
beta-mercaptoethanol 0,1 mM
penicillin 50 U/ml
streptomycin 50 µg/ml
human b-FGF 10 ng/ml

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
TRA 1-81
Yes
SOX2
Yes
c-MYC
Yes
KLF4
Yes
OCT3/4
Yes
REX1
Yes
TDGF1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
PDX1
Yes
SOX7
Yes
AFP
Yes
Protocol or reference
Moretti et al_2010_New Engl J Med_Patient-specific induced pluripotent stem-cell models for long-QT syndrome_appendixSupFig2.pdf
Up-regulation of lineage markers representative of the three embryonic germ layers in induced pluripotent stem cell(iPSC) clones from control and LQT1 individuals (C25 iPSC come from 1 of the 2 Control individuals).
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
CD31
Yes
DES
Yes
ACTA2
Yes
MYL2
Yes
CDH5
Yes
SCL
Yes
Protocol or reference
Moretti et al_2010_New Engl J Med_Patient-specific induced pluripotent stem-cell models for long-QT syndrome_appendixSupFig2.pdf
Up-regulation of lineage markers representative of the three embryonic germ layers in induced pluripotent stem cell(iPSC) clones from control and LQT1 individuals (C25 iPSC come from 1 of the 2 Control individuals).
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
KRT14
Yes
NCAM1
Yes
TH
Yes
GABRR2
Yes
Protocol or reference
Moretti et al_2010_New Engl J Med_Patient-specific induced pluripotent stem-cell models for long-QT syndrome_appendixSupFig2.pdf
Up-regulation of lineage markers representative of the three embryonic germ layers in induced pluripotent stem cell(iPSC) clones from control and LQT1 individuals (C25 iPSC come from 1 of the 2 Control individuals).

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Unknown

Other Genotyping (Cell Line)