C25
TUMi001-A
General
Cell Line |
|
| hPSCreg name | TUMi001-A |
| Cite as: | TUMi001-A (RRID:CVCL_VF08) |
| Alternative name(s) |
C25
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
MRIi001-A (C6) MRIi001-A-1 (C6-AAVS1-iCasRx) |
| Last update | 20th January 2021 |
| User feedback | |
Provider |
|
| Generator |
Technische Universität München (TUM)
Contact:
Technische Universität München (TUM) |
| Owner | Technische Universität München (TUM) |
| Derivation country | Germany |
External Databases |
|
| BioSamples | SAMEA104132875 |
| Cellosaurus | CVCL_VF08 |
| Wikidata | Q54973375 |
General Information |
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| Publications | |
| Projects | |
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
|
| Sex | female |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA104132876 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | No |
| Is there other documentation provided to the donor for consenting purposes? | No |
| Confirm that consent was obtained by a qualified professional | Yes |
| Has the donor agreed to be re-contacted? | Unknown |
| Has the donor been informed about how her/his data will be protected? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| * Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| * Does consent pertain to a specific research project? | No |
| Does consent permit unforeseen future research, without further consent? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | No information |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Ethikkommission der Fakultät für Medizin der Technischen Universität München |
| Approval number | 2109/08 |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
| Name of accrediting authority involved? | Ethikkommission der Fakultät für Medizin der Technischen Universität München |
| Approval number | 2109/08 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
|
| Source cell type |
Any skin fibroblast that is part of some dermis.
|
| Source cell origin |
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
|
Reprogramming method |
|
| Vector type | Integrating |
| Vector | Virus (Retrovirus) |
| Genes | |
| Is the used vector excisable? |
Yes |
| Absence of reprogramming vector(s)? |
No |
| Reprogramming vectors silenced? |
Yes |
| Methods used |
Immunostaining, PCR, RT-PCR
|
| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
|
| Type of used vector free reprogramming factor(s) |
None
|
Other |
|
| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Medium |
Other medium:
Base medium: DMEM/F12
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
|
Characterisation
Analysis of Undifferentiated Cells
Differentiation Potency
In vitro spontaneous differentiation
Protocol or reference
Moretti et al_2010_New Engl J Med_Patient-specific induced pluripotent stem-cell models for long-QT syndrome_appendixSupFig2.pdf
Up-regulation of lineage markers representative of the three embryonic germ layers in induced pluripotent stem cell(iPSC) clones from control and LQT1 individuals (C25 iPSC come from 1 of the 2 Control individuals).
In vitro spontaneous differentiation
Protocol or reference
Moretti et al_2010_New Engl J Med_Patient-specific induced pluripotent stem-cell models for long-QT syndrome_appendixSupFig2.pdf
Up-regulation of lineage markers representative of the three embryonic germ layers in induced pluripotent stem cell(iPSC) clones from control and LQT1 individuals (C25 iPSC come from 1 of the 2 Control individuals).
In vitro spontaneous differentiation
Protocol or reference
Moretti et al_2010_New Engl J Med_Patient-specific induced pluripotent stem-cell models for long-QT syndrome_appendixSupFig2.pdf
Up-regulation of lineage markers representative of the three embryonic germ layers in induced pluripotent stem cell(iPSC) clones from control and LQT1 individuals (C25 iPSC come from 1 of the 2 Control individuals).
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Unknown
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Other Genotyping (Cell Line) |
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