MSHRIe001-A

CA1

General#

Cell Line

hPSCreg Name MSHRIe001-A
Alternative name(s)
CA1
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator Mount Sinai Hospital (MSHRI)
Contact:
Nagy's Lab
Derivation country Canada

External Databases

Cellosaurus CVCL_B844
NIHhESC NIHhESC-11-0137

General Information

Publications
Is the cell line available in principle?
No

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics#

We are currently revising this section.

hESC Derivation#

Cell isolation Immunosurgery

Culture Conditions#

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
Passage method Enzymatically
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 8 %
Supplements
bFGF %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3B
Yes
GABRB3
Yes
GDF3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
SOX2
Yes
TDGF1
Yes
SSEA-1
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY

Other Genotyping (Cell Line)