CA1
MSHRIe001-A
General#
Cell Line |
|
| hPSCreg Name | MSHRIe001-A |
| Alternative name(s) |
CA1
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| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
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| Generator |
Mount Sinai Hospital (MSHRI)
Contact:
Nagy's Lab |
| Derivation country | Canada |
External Databases |
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| Cellosaurus | CVCL_B844 |
| NIHhESC | NIHhESC-11-0137 |
| CLO | CLO_0100200 |
| Wikidata | Q54906847 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
No |
Donor Information#
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics#
| Alternatives to consent | |
| Alternative consent approval number | NIHhESC-11-0137 |
hESC Derivation#
| Cell isolation | Immunosurgery |
Culture Conditions#
| Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
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| Passage method | Enzymatically | ||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 8 % Supplements
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Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| DNMT3B |
Yes |
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| GABRB3 |
Yes |
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| GDF3 |
Yes |
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| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| SOX2 |
Yes |
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| TDGF1 |
Yes |
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| SSEA-1 |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XY
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Other Genotyping (Cell Line) |
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