CA1
MSHRIe001-A
General#
Cell Line |
|
| hPSCreg Name | MSHRIe001-A |
| Alternative name(s) |
CA1
|
| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
|
| Generator |
Mount Sinai Hospital (MSHRI)
Contact:
Nagy's Lab |
| Distributors | |
| Derivation country | Canada |
External Databases |
|
| Cellosaurus | CVCL_B844 |
| NIHhESC | NIHhESC-11-0137 |
| CLO | CLO_0100200 |
| Wikidata | Q54906847 |
General Information |
|
| Publications |
|
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information#
General Donor Information |
|
| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | |
Ethics#
| Alternatives to consent | |
| Alternative consent approval number | NIHhESC-11-0137 |
hESC Derivation#
| Cell isolation | Immunosurgery |
Culture Conditions#
| Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
||||||
| Passage method | Enzymatically | ||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 8 % Supplements
|
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| DNMT3B |
Yes |
|
||||
| GABRB3 |
Yes |
|
||||
| GDF3 |
Yes |
|
||||
| NANOG |
Yes |
|
||||
| POU5F1 (OCT-4) |
Yes |
|
||||
| ZFP42 (REX-1) |
Yes |
|
||||
| SOX2 |
Yes |
|
||||
| TDGF1 |
Yes |
|
||||
| SSEA-1 |
Yes |
|
||||
| SSEA-3 |
Yes |
|
||||
| SSEA-4 |
Yes |
|
||||
| TRA 1-60 |
Yes |
|
||||
| TRA 1-81 |
Yes |
|
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XY
|
Other Genotyping (Cell Line) |
|
Login to share your feedback, experiences or results with the research community.