CA1
MSHRIe001-A
General
Cell Line |
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hPSCreg Name | MSHRIe001-A |
Alternative name(s) |
CA1
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Cell line type | Human embryonic stem cell (hESC) |
Last update | 22nd May 2019 |
User feedback | |
Provider |
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Generator |
Mount Sinai Hospital (MSHRI)
Contact:
Nagy's Lab |
Derivation country | Canada |
External Databases |
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Cellosaurus | CVCL_B844 |
NIHhESC | NIHhESC-11-0137 |
CLO | CLO_0100200 |
Wikidata | Q54906847 |
General Information |
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Publications |
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* Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases |
Ethics
Alternatives to consent | |
Alternative consent approval number | NIHhESC-11-0137 |
hESC Derivation
Cell isolation | Immunosurgery |
Culture Conditions
Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
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Passage method | Enzymatically | ||||||
Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 8 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
DNMT3B |
Yes |
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GABRB3 |
Yes |
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GDF3 |
Yes |
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NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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ZFP42 (REX-1) |
Yes |
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SOX2 |
Yes |
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TDGF1 |
Yes |
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SSEA-1 |
Yes |
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SSEA-3 |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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TRA 1-81 |
Yes |
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Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46XY
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Other Genotyping (Cell Line) |
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