Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium


In this study, we established induced pluripotent stem (iPS) cell lines from postmortem dura-derived fibroblasts of four control individuals with low polygenic risk score for psychiatric disorders including schizophrenia and bipolar disorder. The fibroblasts were reprogrammed into iPS cells using episomal vectors carrying OCT3/4, SOX2, KLF4, L-Myc, LIN28 and shRNA-p53. All iPS cell lines showed the same genotype with parental postmortem brain tissues, expressed pluripotency markers, and exhibited the differentiation potency into three embryonic germ layers. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Sawada T, Benjamin KJM, Brandtjen AC, Tietze E, Allen SJ, Paquola ACM, Kleinman JE, Hyde TM, Erwin JA
Journal Stem cell research
Publication Date 2020 Jul;46:101806
PubMed 32446240
DOI 10.1016/j.scr.2020.101806

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