General

Cell Line

hPSCreg name IUFi002-A
Cite as:
IUFi002-A (RRID:CVCL_C6Q5)
Cell line type Human induced pluripotent stem cell (hiPSC)
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Last update 23rd January 2023
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Provider

Generator IUF – Leibniz Research Institute for Environmental Medicine (IUF)
Owner IUF – Leibniz Research Institute for Environmental Medicine (IUF)
Derivation country Germany

External Databases

BioSamples SAMEA111528658
Cellosaurus CVCL_C6Q5
Wikidata Q117704563

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Leigh Syndrome (LS)
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • LS
  • LSS
  • Leigh disease
  • Leigh syndrome
  • Leigh syndrome spectrum
  • Leigh's disease
  • infantile necrotizing encephalomyelopathy
  • infantile subacute necrotizing encephalopathy
  • juvenile subacute necrotizing encephalomyelopathy
  • Leigh syndrome due to mitochondrial Complex 1 deficiency
  • Leigh syndrome due to mitochondrial Complex 2 deficiency
  • Leigh syndrome due to mitochondrial Complex 3 deficiency
  • Leigh syndrome due to mitochondrial Complex 4 deficiency
  • Leigh syndrome due to mitochondrial Complex 5 deficiency
  • Leigh's necrotizing encephalopathy
  • SNE
  • necrotizing encephalopathy, infantile Subacute, of Leigh
  • subacute necrotizing encephalomyelopathy
  • subacute necrotizing encephalopathy
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA112358966

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: felix.distelmaier@med.uni-duesseldorf.de
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink felix.distelmaier@med.uni-duesseldorf.de
Confirm that consent was obtained by a qualified professional Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Heinrich-Heine University Duesseldorf
Approval number 2021-1340
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
O2 Concentration 37 %
CO2 Concentration 5 %
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SOX2
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
LIN28
Yes
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
Project_Image005_ch00.tif
Bright field image of IPSC colony (IUFi002-A)
Embryoid bodies (EB) in vitro spontaneous differentiation
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
alpha fetoprotein
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
smooth muscle actin
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
nestin
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)