General

Cell Line
hPSCreg name	ICHi003-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	ICHi003-A
Alternative name(s)	TTNtv-003
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	No similar lines found.
Last update	20th January 2025
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Provider
Generator	IRCCS Istituto Clinico Humanitas (ICH)
External Databases
BioSamples	SAMEA117610351
General Information
* Is the cell line readily obtainable for third parties?	No

Donor Information

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Cardiomyopathy, Dilated The donor is a carrier of a disease-associated mutation and affected. Genetic variants TTN (target) Chromosome location 2q31.2 Nucleotide sequence variant in HGVS format NM_003319.4:c.57847delGTAA
Disease associated phenotypes	dilated cardiomyopathy

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived?	Yes
Was the consent voluntarily given?	Yes
Has the donor been informed that participation will not directly influence their personal treatment?	Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor?	Yes
Do you (Depositor/Provider) hold the original Donor Consent Form?	Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised.	pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells?	Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world?	No
How may genetic information associated with the cell line be accessed?	No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample?	No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?	Yes
Name of accrediting authority involved?	Review board ICH and ASUGI CERU
Approval number	ID:1215 and 43_2009
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
Source cell type	Peripheral Blood Mononuclear Cell A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes. Synonyms PERIPHERAL BLOOD MONONUCLEAR CELL Peripheral Blood Mononuclear Cell PBMC
Reprogramming method
Vector type	Non-integrating
Vector	Sendai virus
Genes	POU5F1 SOX2 KLF4 MYC
Is reprogramming vector detectable?	No
Methods used	PCR
Vector free reprogramming
Other
Derived under xeno-free conditions	Unknown
Derived under GMP?	Unknown
Available as clinical grade?	Unknown

Culture Conditions

Medium

Other medium: Base medium: Stem Flex Main protein source: Stem Flex Supplement Serum concentration: 0 % Supplements Supplement Amount Unit Stem Flex Supplement 10 %

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
SSEA-4	Yes	–
TRA 1-60	Yes	–

Morphology

Morphology pictures

Differentiation Potency

Endoderm

Mesoderm

Ectoderm

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46, XY Passage number: 10 Karyotyping method: G-Banding
Other Genotyping (Cell Line)