Generation of induced pluripotent stem cell line NIMHi010-A from dermal fibroblast cells of a healthy individual
Summary
In this study, we have established human induced pluripotent stem cell (hiPSC) line, NIMHi010-A of a 42-year-old healthy donor. The iPSC line was generated from human dermal fibroblasts using Sendai viruses carrying reprogramming factors c-MYC, SOX2, KLF4, and OCT4 under a feeder-free culture system. The generated hiPSC line expressed typical pluripotency markers, displayed a normal karyotype, and demonstrated the potential to differentiate into the three germ layers. This hiPSC line will serve as a healthy control model for physiological processes and drug screening of Asian origin from Indian population. Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Dash SS, Arunachal G, Nimonkar MM, Vengalil S, Nashi S, Chetan GK, Boddu VK, Nalini A, Markandeya YS |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Apr;76:103355 |
| PubMed | 38412659 |
| DOI | 10.1016/j.scr.2024.103355 |