RC-8, RC8
RCe012-A
General
Cell Line |
|
| hPSCreg name | RCe012-A |
| Cite as: | RCe012-A (RRID:CVCL_C790) |
| Alternative name(s) |
RC-8, RC8
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 28th November 2017 |
| User feedback | |
Provider |
|
| Generator | Roslin Cells (RC) |
| Owner | Derivation of Clinical Grade hES Cells |
| Distributors | |
| Derivation country | United Kingdom |
External Databases |
|
| Cellosaurus | CVCL_C790 |
| Wikidata | Q54949412 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | |
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | HFEA |
hESC Derivation
| Date of derivation | 2009-07-23 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| ZP removal technique | Mechanical |
| Cell isolation | None |
| Cell seeding | Isolated ICM |
Culture Conditions
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| SSEA-1 |
No |
|
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 11
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Other Genotyping (Cell Line) |
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