MstrShef3, MShef3, MasterShef3
UOSe008-A
General
Cell Line |
|
| hPSCreg name | UOSe008-A |
| Cite as: | UOSe008-A (RRID:CVCL_BW76) |
| Alternative name(s) |
MstrShef3, MShef3, MasterShef3
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 2nd May 2022 |
| User feedback | |
Provider |
|
| Generator |
University of Sheffield (UOS)
Contact:
Centre for Stem Cell Biology |
| Owner | Centre for Stem Cell Biology |
| Distributors | |
| Derivation country | United Kingdom |
External Databases |
|
| Cellosaurus | CVCL_BW76 |
| BioSamples | SAMEA104627961 |
| Wikidata | Q54904049 |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
|
Donor Information
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Family history | No |
| Is the medical history available upon request? | No |
| Is clinical information available? | No |
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
No
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA104627962 |
Ethics
| Was the embryo established purely for research purposes? | No |
| Have both parents consented to the use of the embryo for ESC derivation? | Yes |
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | Yes |
| Please provide the contact information | https://www.carefertility.com/our-clinics/ivf-clinic-nottingham/ |
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Alternative consent approval number | |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | No information |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | National Research Ethics Service - North West London Rec-2 |
| Approval number | 05/Q0501/63 |
hESC Derivation
| Date of derivation | 2011-08-12 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
| PGD Embryo? |
No |
| Expansion status |
3
|
| ICM morphology |
B
|
| Trophectoderm morphology |
b
|
| ZP removal technique | Laser |
| Cell isolation | None |
| Cell seeding | Embryo |
| Derived under xeno-free conditions? |
No |
| Derivation under GMP? |
Yes |
| Available as clinical grade? |
Yes |
Culture Conditions
| Surface coating | Non coated |
| Feeder cells |
Human Foreskin Fibroblasts Cellfinder Ont Id: CELDA_000001419 |
| Passage method | Mechanically |
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: KO-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
|
||||
| POU5F1 (OCT-4) |
Yes |
|
||||
| SSEA-3 |
Yes |
|
|
|||
| TRA 1-81 |
Yes |
|
|
|||
| SSEA-4 |
Yes |
|
||||
| TRA 1-60 |
Yes |
|
Morphology pictures
Cells in culture following thaw from preMCB
Differentiation Potency
In vitro spontaneous differentiation
| Marker | Expressed |
| alpha fetal protein |
No |
In vitro spontaneous differentiation
| Marker | Expressed |
| Beta Tubulin III |
Yes |
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Certificate of Analysis |
|
| Is there a certificate of analysis available? |
Yes
Passage:
|
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46, XX [50]
Passage number: +5
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |
|
Login to share your feedback, experiences or results with the research community.