Viral-free generation and characterization of a human induced pluripotent stem cell line from dermal fibroblasts


Peripheral dermal fibroblasts (DF) from a healthy 56 year old female were obtained from the Centre for Healthy Brain Ageing (CHeBA) Biobank, University of New South Wales, under the material transfer agreement with the University of Wollongong. DFs were reprogrammed via mRNA-delivered transcription factors into induced pluripotent stem cells (iPSCs). The generated iPSCs were confirmed to be pluripotent, capable of three germ layer differentiation and are thus a useful resource for creating iPSC-derived healthy human cells of any lineage. Resource table. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Engel M, Balez R, Muñoz SS, Cabral-da-Silva MC, Stevens CH, Bax M, Do-Ha D, Sidhu K, Sachdev P, Ooi L
Journal Stem cell research
Publication Date 2018 Oct;32:135-138
PubMed 30278375
DOI 10.1016/j.scr.2018.09.014

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