CSIR-SA-001-L2
CSIRi002-A
General
Cell Line |
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| hPSCreg name | CSIRi002-A |
| Cite as: | CSIRi002-A (RRID:CVCL_E3U1) |
| Alternative name(s) |
CSIR-SA-001-L2
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 2nd September 2024 |
| Notes | Derived from Lonza dermal fibroblasts (Cat# CC-2511, 23801, lot 0000268030) |
| User feedback | |
Provider |
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| Generator | Council for Scientific and Industrial Research South Africa (CSIR) |
| Distributors | |
| Derivation country | South Africa |
External Databases |
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| BioSamples | SAMEA115883343 |
| Cellosaurus | CVCL_E3U1 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Black or African American |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA115889096 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | No |
| Alternatives to consent are available? | Yes |
| Alternatives to consent | Certificate of analysis from Lonza |
| Alternative consent approval number | |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | No |
| * Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | No information |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | CSIR REC |
| Approval number | 17_2013 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell line name | Lonza cat no. CC-2511 NHDF 23801 Lot No. 0000268030 |
| Source cell type |
Synonyms
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| Passage number reprogrammed | 15 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Methods used |
RT-PCR
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Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Passage method |
Enzymatically
TrypLE
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| Medium |
Essential 8™
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| NANOG |
Yes |
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| SOX2 |
Yes |
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Morphology pictures
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46, XY
Passage number: 15
Karyotyping method:
Molecular karyotype (NGS)
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Other Genotyping (Cell Line) |
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