Generation of a patient-specific iPSC cell line with cardiac arrhythmias and dilated cardiomyopathy (CBRCULi016-A), an isogenic control (CBRCULi016-A-1), and a paternal control (CBRCULi017-A)
Summary
Dilated cardiomyopathy (DCM) is a prevalent cause of heart failure. We generated induced pluripotent stem cell (iPSC) lines from a DCM patient carrying a mutation in the SCN5A gene, with his healthy father serving as a control. Notably, we employed CRISPR-Cas9 to rectify the mutation in the patient's iPSC line. The resulting iPSC lines expressed pluripotency markers, underwent differentiation into all three embryonic germ layers, maintained a normal karyotype, and lacked reprogramming viral vectors. These iPSC lines serve as a model for delving into the mechanisms of DCM and hold promise for the development of personalized therapeutic approaches. Copyright © 2024. Published by Elsevier B.V.
| Authors | Djemai M, Jauvin D, Poulin H, Chapotte-Baldacci CA, Chahine M |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Mar;75:103308 |
| PubMed | 38232626 |
| DOI | 10.1016/j.scr.2024.103308 |