LUMC0004iCTRL10

General#

Cell Line

hPSCreg Name LUMCi029-B
Alternative name(s)
LUMC0004iCTRL10
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 31st March 2020
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Provider

Generator Leiden University Medical Center (LUMC)
Owner LUMC hiPSC core facility
Distributors
Derivation country Netherlands

External Databases

BioSamples SAMEA6878157
Cellosaurus CVCL_ZA10
CLO CLO_0100741

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: allowed
Additional restrictions:

For commercial users a licence for the reprogramming vectors is neccecery

Donor Information#

General Donor Information

Sex male
Ethnicity unknown

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history N/A
Is the medical history available upon request? No
Is clinical information available? No

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA6673739

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? No
Was the consent voluntarily given? No
Has the donor been informed that participation will not directly influence their personal treatment? No
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: NA
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? No
If you do not hold the SIGNED Donor Consent Form, do you know who does? No
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? No
Alternatives to consent are available? Yes
Alternatives to consent Regulations LUMC regarding the use of surgical waste material for research (in Dutch)
Alternative consent approval number
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional No
Has the donor agreed to be re-contacted? Unknown
Has the donor been informed about how her/his data will be protected? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Leiden University Medical Center Ethical Commitee
Approval number Paraplu 13080
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? Yes
Further constraints on use For commercial use a license for the reprogramming vectors is required.
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Christopher Baum Hannover Medical School, Germany
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? Yes
Constraints for use or distribution For commercial use a license for the reprogramming vectors is required.

hIPSC Derivation#

General

Source cell type
skin fibroblast
Source cell origin
Skin
An organ that constitutes the external surface of the body. It consists of the epidermis, dermis, and skin appendages.
Synonyms
  • Skin
  • Human Skin
  • SKIN
  • Integument
  • Skin, total
  • Skin, NOS
show more synonyms

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Genes
Is the used vector excisable?
Yes
Absence of reprogramming vector(s)?
No
Reprogramming vectors silenced?
Unknown
Notes on reprogramming vector silencing In addition to OSKM lentivirus carries a tomato signal which can be used to check transgene silencing if necessary.
Vector map

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-81
Yes
POU5F1 (OCT-4)
Yes
NANOG
Yes
SOX2
Yes
SSEA-3
Yes
SSEA-4
Yes
Pluripotency Score Novelty Score
22.49 1.19
Morphology pictures
LUMC0004iCTRL10 cultured on VN/TeSR-plus. p14. 40x
LUMC0004iCTRL10 cultured on VN/TeSR-plus. p14. 100x
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
alpha fetoprotein (AFP)
Yes
Protocol or reference
LUMC0004iCTRL10 spondiff.pdf
Spontaneous Diff of LUMC0004iCTRL10 p11
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
PECAM1(CD31)
Yes
Protocol or reference
LUMC0004iCTRL10 spondiff.pdf
Spontaneous Diff of LUMC0004iCTRL10 p11
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
beta-III tubulin
Unknown
Protocol or reference
LUMC0004iCTRL10 spondiff.pdf
Spontaneous Diff of LUMC0004iCTRL10 p11

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Passage number: 27
Karyotyping method: G-Banding

Other Genotyping (Cell Line)