homoHbCS iPSC, MU018

General

Cell Line

hPSCreg Name
MUi034-A
Alternative name(s)
homoHbCS iPSC, MU018
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 29th September 2022
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Provider

Generator Mahidol University (MU)

External Databases

Cellosaurus CVCL_B7E8
Wikidata Q112930143
BioSamples SAMEA111396563

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
alpha thalassemia
The donor is affected.
Stage
Mild anemia

External Databases (Donor)

BioSamples SAMEA111396677

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Research Ethic Committee No.4 Faculty of Medicine, Chiang Mai University
Approval number PED-2564-08538
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Hematopoietic Stem Cells

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium Essential 8™

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
TDGF1
Yes
DNMT3B
Yes
GABRB3
Yes
GDF3
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
Sox17
Yes
AFP
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
TBXT
Yes
SMA
Yes
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
NEUROD1
Yes
TUJ1
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal female karyotype (46, XX)
Karyotyping method: G-Banding

Other Genotyping (Cell Line)