Generation of a human induced pluripotent stem cell line (HMGUi002-A) from a healthy male individual


Induced pluripotent stem cells (iPSCs) can be used to generate different somatic cell types in vitro, including insulin-producing pancreatic β-cells. Here, we have generated iPSCs from a healthy male individual using an episomal reprogramming method. The resulting iPSCs are integration-free, have a normal karyotype and are pluripotent in vitro and in vivo. Furthermore, we show that this iPSC line can be differentiated into pancreatic lineage cells. Taken together, this iPSC line will be useful to test differentiation protocols towards β-cell as well as other cell types and will also serve as a control for drug development and disease modelling studies. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Wang X, Malinowski AR, Beckenbauer J, Siehler J, Blöchinger A, Meitinger T, Häring HU, Staiger H, Burtscher I, Lickert H
Journal Stem cell research
Publication Date 2019 Aug;39:101531
PubMed 31419739
DOI 10.1016/j.scr.2019.101531

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