Establishment of SIAISi002-A, an induced pluripotent stem cell (iPSC) line from 39-year old healthy female donor with a family history of Alzheimer's disease (AD) and two copies of APOE4 gene

Summary

A 39-year old healthy female with a family history of Alzheimer's disease (AD) and two copies of apolipoprotein E(APOE) ε4 allele donated her Peripheral blood mononuclear cells (PBMC). The non-integrative Sendai viral vectors used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of iPSCs was confirmed by immunocytochemistry and ability of differentiation spontaneously into 3 germ layers. Furthermore, the iPSC line displayed a normal karyotype. The APOE gene is currently considered to be the most relevant gene for sporadic AD. Our model might offer a platform to study the pathological mechanisms and drug testing studies in AD. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Yan Y, Qiu Y, Zhao A, Zhao J, Wang Y, Deng Y
Journal Stem cell research
Publication Date 2020 Apr 22;45:101792
PubMed 32339902
DOI 10.1016/j.scr.2020.101792

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