CBiPS1sv-4F-40

General#

Cell Line

hPSCreg Name ESi007-A
Alternative name(s)
CBiPS1sv-4F-40
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
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Provider

Generator Spanish Stem Cell Bank (ES)
Contact:
Inbiomed
Owner Inbiomed
Distributors
Derivation country Spain

External Databases

BioSamples SAMEA3303231
Cellosaurus CVCL_V189
EBiSC ESi007-A
ECACC 66540070

General Information

Projects
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA3303248

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
cord blood hematopoietic stem cell
Any hematopoietic stem cell that is part of a umbilical cord blood.
Source cell origin
umbilical cord blood
blood that remains in the placenta and in the attached umbilical cord after childbirth[WP].
Source cell type (free text) Cord blood CD133+
Age of donor (at collection) neonate

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Selection criteria for clones morphological
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Gelatin
Feeder cells Mouse embryonic fibroblasts
Cellfinder Ont Id: EFO_0004040
Passage method Mechanically
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
NEAA 1 %
Glutamax 2 mM
2-mercaptoethanol 0,05 %
bFGF 10 ng/ml
Penicillin Streptomycin 0,5 %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
TRA 1-60
Yes
ZFP42 (REX-1)
Yes
SOX2
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
FOXA2
Yes
AFP
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
SMA
Yes
MEOX
Yes
AAP
Yes
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
Tuj1
Yes
PAX6
Yes
NeuroD3
Yes
Sox1
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes