Induction of Bile Canaliculi-Forming Hepatocytes from Human Pluripotent Stem Cells
Summary
Cell polarity and formation of bile canaliculi can be achieved in hepatocytes which are generated from patient-derived induced pluripotent stem cells. This allows for the study of endogenous mutant proteins, patient-specific pathogenesis, and drug responses for diseases where hepatocyte polarity and bile canaliculi play a key role. Here, we describe a step-by-step protocol for the generation of bile canaliculi-forming hepatocytes from induced pluripotent stem cells and their evaluation. © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
| Authors | Matakovic L, Overeem AW, Klappe K, van IJzendoorn SCD |
|---|---|
| Journal | Methods in molecular biology (Clifton, N.J.) |
| Publication Date | 2022;2544:71-82 |
| PubMed | 36125710 |
| DOI | 10.1007/978-1-0716-2557-6_4 |