NCL-9

General#

Cell Line

hPSCreg Name UNEWe009-A
Alternative name(s)
NCL-9
Cell line type Human embryonic stem cell (hESC)
Last update 10th April 2017
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Provider

Generator University of Newcastle (UNEW)
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_A010

General Information

Publications

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics#

Alternatives to consent
Name of accrediting authority involved?
Approval number

hESC Derivation#

Embryo stage Morula
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Chemical
Cell seeding Embryo

Culture Conditions#

Surface coating Gelatin
Feeder cells Swiss MF-1 Mice
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
Medium conditioned by hES-NCL1 cells %
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
NANOG
Yes
ZFP42 (REX-1)
Yes
TERT
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46 XX

Other Genotyping (Cell Line)