Generation of a SIAISi004-A hiPSC line from PBMCs of a 74 year-old Alzheimer's disease woman by non-integrating sendai virus mediated reprogramming


A 74-year-old Alzheimer's Disease (AD) woman patient donated her peripheral blood mononuclear cells (PBMC). Non-integrating episomal vector system were used to reprogram their PBMCs with human OKSM (OCT3/4, KLF4, SOX2, and c-MYC) transcription factors. Immunocytochemistry and flow cytometry for pluripotency makers confirmed the pluripotency of transgene-free iPSCs. Pluripotency was confirmed by the ability of iPSCs to spontaneously differentiate three germ layers in vitro as well. The newly generated human iPSC from PBMCs of a 74 year-old Alzheimer's disease woman can be used for studying further disease mechanisms of Alzheimer's Disease (AD). Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Zhang W, Di W, Zhao J, Zhang B, Wang Y
Journal Stem cell research
Publication Date 2021 Aug;55:102501
PubMed 34450529
DOI 10.1016/j.scr.2021.102501

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