ERA-3
ULBe003-A
General
Cell Line |
|
| hPSCreg name | ULBe003-A |
| Cite as: | ULBe003-A (RRID:CVCL_9007) |
| Alternative name(s) |
ERA-3
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 28th July 2017 |
| User feedback | |
Provider |
|
| Generator | Université Libre de Bruxelles (ULB) |
| Derivation country | Belgium |
External Databases |
|
| Cellosaurus | CVCL_9007 |
| Wikidata | Q54990724 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | |
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Federal Commission for Medical and Scientific Research on embryos in vitro |
| Approval number |
hESC Derivation
| Date of derivation | 2005-11-10 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| ZP removal technique | Chemical |
| Cell isolation | Mechanical |
| Cell seeding | Embryo |
Culture Conditions
| Surface coating | Gelatin | |||||||||
| Passage method | Mechanically | |||||||||
| O2 Concentration | 20 % | |||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 15 % Supplements
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-1 |
No |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| hTERT |
Yes |
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| NANOG |
Yes |
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| ZFP42 (REX-1) |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 46
|
Other Genotyping (Cell Line) |
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