f3SMA3
ICGi003-A
General
Donor Information
General Donor Information |
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Sex | female |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
Unknown
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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BioSamples | SAMEA6877530 |
Ethics
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
Was the consent voluntarily given? | Yes |
Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
How may genetic information associated with the cell line be accessed? | Controlled Access |
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
Name of accrediting authority involved? | the D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology ethics committee |
Approval number | 65/2014 |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
Name of accrediting authority involved? | the D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology ethics committee |
Approval number | 65/2014 |
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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Source cell line name | f3SMA |
Source cell type |
A leukocyte with a single non-segmented nucleus in the mature form.
Synonyms
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Source cell origin |
Body substance which consists of plasma and blood cells
Synonyms
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Passage number reprogrammed | 6 |
Reprogramming method |
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Vector type | Non-integrating |
Vector | Episomal |
Genes | |
Is reprogramming vector detectable? |
No |
Methods used |
PCR
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Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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Other |
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Selection criteria for clones | ES-like morphology |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions
Surface coating | Gelatin | |||||||||||||||||||||
Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
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Passage method |
Enzymatically
TrypLE
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CO2 Concentration | 5 % | |||||||||||||||||||||
Medium |
Other medium:
Base medium: KO-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 15 % Supplements
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
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Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
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Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
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NANOG |
Yes |
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SOX2 |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
Score:
Marker | Present | Absent |
mCpG | ||
OCT4 |
Differentiation Potency
In vitro spontaneous differentiation
Marker | Expressed |
AFP |
Yes |
In vitro spontaneous differentiation
Marker | Expressed |
NEFH |
Unknown |
Microbiology / Virus Screening |
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Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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