Generation and characterization of three induced pluripotent stem cell lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) from a 51-year-old healthy individual
Summary
Skin punch biopsy was donated by a healthy 51-year-old Caucasian male and the dermal fibroblasts were reprogrammed into human induced pluripotent stem cell (hiPSC) lines by using non-integrative Sendai viruses expressing OCT4, SOX2, KLF4 and c-MYC. Three iPSC lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) highly expressed the pluripotent markers and were capable of differentiating into cells of endodermal, mesodermal, and ectodermal origin. These iPSCs can be offered as controls and in combination with genome-editing and three-dimensional (3D) system. They may be used for human disease modelling and drug screening. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Liu M, Ge N, Han Y, Reilly J, Yang M, Yang F, Krawczyk J, McInerney V, O'Brien T, Prendiville T, Shen S |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 Dec;57:102607 |
| PubMed | 34844101 |
| DOI | 10.1016/j.scr.2021.102607 |