Generation and characterization of three induced pluripotent stem cell lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) from a 51-year-old healthy individual


Skin punch biopsy was donated by a healthy 51-year-old Caucasian male and the dermal fibroblasts were reprogrammed into human induced pluripotent stem cell (hiPSC) lines by using non-integrative Sendai viruses expressing OCT4, SOX2, KLF4 and c-MYC. Three iPSC lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) highly expressed the pluripotent markers and were capable of differentiating into cells of endodermal, mesodermal, and ectodermal origin. These iPSCs can be offered as controls and in combination with genome-editing and three-dimensional (3D) system. They may be used for human disease modelling and drug screening. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Liu M, Ge N, Han Y, Reilly J, Yang M, Yang F, Krawczyk J, McInerney V, O'Brien T, Prendiville T, Shen S
Journal Stem cell research
Publication Date 2021 Nov 24;57:102607
PubMed 34844101
DOI 10.1016/j.scr.2021.102607

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