Generation and characterization of three induced pluripotent stem cell lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) from a 51-year-old healthy individual
Skin punch biopsy was donated by a healthy 51-year-old Caucasian male and the dermal fibroblasts were reprogrammed into human induced pluripotent stem cell (hiPSC) lines by using non-integrative Sendai viruses expressing OCT4, SOX2, KLF4 and c-MYC. Three iPSC lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) highly expressed the pluripotent markers and were capable of differentiating into cells of endodermal, mesodermal, and ectodermal origin. These iPSCs can be offered as controls and in combination with genome-editing and three-dimensional (3D) system. They may be used for human disease modelling and drug screening. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.
|Authors||Liu M, Ge N, Han Y, Reilly J, Yang M, Yang F, Krawczyk J, McInerney V, O'Brien T, Prendiville T, Shen S|
|Journal||Stem cell research|
|Publication Date||2021 Nov 24;57:102607|