HuES14

General#

Cell Line

hPSCreg Name HVRDe014-A
Alternative name(s)
HuES14
Cell line type Human embryonic stem cell (hESC)
Last update 24th August 2017
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Provider

Generator Harvard University (HVRD)
Contact:
Melton Lab
Derivation country United States

External Databases

Cellosaurus CVCL_B144
NIHhESC NIHhESC-09-0027

General Information

Publications
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics#

We are currently revising this section.

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
Expansion status 4
Stephenson 2007
ICM morphology A
Stephenson 2007
Trophectoderm morphology a
Stephenson 2007
ZP removal technique Chemical
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions#

Passage method Enzymatically
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 10 %
Supplements
bFGF %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
DNMT3B
Yes
GABRB3
Yes
GDF3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
TDGF1
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)