Development and characterization of human iPSC line NCCSi004-A from umbilical cord blood (UCB) derived CD34(+)cells obtained from donor belonging to Indian ethnic population
Summary
Here we report the reprogramming of CD34+ cells obtained from UCB of a healthy donor female child belonging to the Indian ethnic population. These CD34+cells were subjected to nucleofection for delivery of episomal vectors expressing Oct4, Sox2, L-Myc, Lin28, Klf4 and p53DD (negative mutation in p53). The iPSC colonies expressed pluripotency markers as detected by PCR, immunofluorescence and flow-cytometry. The removal of plasmid was confirmed by its absence in cells at higher passages. Karyotype analysis revealed a stable genome. The property of in vitro differentiation to tri-lineage was confirmed by expression of markers by immunofluorescence. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Fernandes S, Tembe S, Singh S, Vardhan S, Nair V, Kale V, Limaye L |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2019 Mar;35:101392 |
| PubMed | 30711803 |
| DOI | 10.1016/j.scr.2019.101392 |