Development and characterization of human iPSC line NCCSi004-A from umbilical cord blood (UCB) derived CD34+cells obtained from donor belonging to Indian ethnic population
Here we report the reprogramming of CD34+ cells obtained from UCB of a healthy donor female child belonging to the Indian ethnic population. These CD34+cells were subjected to nucleofection for delivery of episomal vectors expressing Oct4, Sox2, L-Myc, Lin28, Klf4 and p53DD (negative mutation in p53). The iPSC colonies expressed pluripotency markers as detected by PCR, immunofluorescence and flow-cytometry. The removal of plasmid was confirmed by its absence in cells at higher passages. Karyotype analysis revealed a stable genome. The property of in vitro differentiation to tri-lineage was confirmed by expression of markers by immunofluorescence. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.
|Authors||Fernandes S, Tembe S, Singh S, Vardhan S, Nair V, Kale V, Limaye L|
|Journal||Stem cell research|
|Publication Date||2019 Mar;35:101392|