hAdMSiPS1

General#

Cell Line

hPSCreg Name KSCBi003-A
Alternative name(s)
hAdMSiPS1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 29th March 2018
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Provider

Generator National Stem Cell Bank (KSCB)
Distributors
Derivation country South Korea

External Databases

BioSamples SAMEA4675624
Cellosaurus CVCL_VE04

General Information

Publications
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex female
Ethnicity Korean

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46, XX
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA4675623

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
adipose tissue derived mesenchymal stem cell
A adult mesenchymal stem cell derived from adipose tisssue
Synonyms
  • human mesenchymal stem cell from adipose tissue
  • hMSC-AT
Source cell origin
adipose tissue
Portion of connective tissue composed of adipocytes enmeshed in areolar tissue
Synonyms
  • fat
  • fatty tissue
  • fat tissue

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
Yes
Methods used
PCR
Notes on reprogramming vector detection reverse transcription PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Medium Other medium:
Base medium: DMDM/F12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
non-essential amino acids solution 0.1 mmol/L mM
Beta-mercaptoethanol 0.1mmol/L mM
penicillin-streptomycin 1 %
MycoZap plus-PR 0.1 %
basic fibroblast growth factor 4 ng/ml
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)