Generation of induced pluripotent stem cell line (VRISGi004-A) from a healthy female donor by reprogramming erythroid progenitor cells
Summary
We generated a human induced pluripotent stem cell (hiPSC) line from erythroid progenitor cells (EPCs) of a 20-year-old female healthy donor using Sendai virus vector encoding Yamanaka factors OCT3/4, SOX2, c-MYC, and KLF4. The established hiPSCs showed a standard morphology and expression of typical undifferentiated stem cell markers, a normal karyotype (46, XX), and demonstrated potential for differentiation in vitro. Furthermore, they were successfully differentiated into cardiomyocytes that expressed cardiomyocyte-specific markers. The iPSC line and iPSC-derived cardiomyocytes will provide new avenues for future drug testing/development and personalized cell therapy for cardiovascular diseases (CVDs). Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Luong TH, Pham TA, Nhung Nguyen TH, Nguyen XH |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Apr;76:103331 |
| PubMed | 38341988 |
| DOI | 10.1016/j.scr.2024.103331 |