ERY02-hiPSC-CL
VRISGi004-A
General
Cell Line |
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hPSCreg name | VRISGi004-A |
Cite as: | VRISGi004-A (RRID:CVCL_D0PG) |
Alternative name(s) |
ERY02-hiPSC-CL
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines | No similar lines found. |
Last update | 16th February 2024 |
User feedback | |
Provider |
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Generator | Vinmec Research Insitute of Stem Cell and Gene Technology (VRISG) |
Owner | Vinmec Research Insitute of Stem Cell and Gene Technology (VRISG) |
Distributors | |
Derivation country | Vietnam |
External Databases |
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BioSamples | SAMEA113597710 |
Cellosaurus | CVCL_D0PG |
Wikidata | Q123033773 |
General Information |
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Publications | |
* Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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Sex | female |
Age of donor (at collection) | 20-24 |
Ethnicity | 20 |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Disease associated phenotypes | no phenotypes |
Family history | N/A |
Is the medical history available upon request? | N/A |
Is clinical information available? | N/A |
Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
Yes
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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BioSamples | SAMEA113601382 |
Ethics
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
Was the consent voluntarily given? | Yes |
Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
Please provide contact information of the holder of the original Donor Information Sheet. | v.nhungnth46@vinmec.com |
Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
Alternatives to consent are available? | No |
Is there other documentation provided to the donor for consenting purposes? | No |
Confirm that consent was obtained by a qualified professional | Yes |
Has the donor agreed to be re-contacted? | Yes |
Has the donor been informed about how her/his data will be protected? | Yes |
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
Does consent expressly prevent the derivation of pluripotent stem cells? | No |
Does consent pertain to a specific research project? | No |
Does consent permit unforeseen future research, without further consent? | Yes |
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? | Yes |
Does consent expressly prevent development of commercial products? | No |
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? | No |
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? | No |
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? | No |
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
Does consent permit research by | |
an academic institution? | Yes |
a public organisation? | Yes |
a non-profit company? | Yes |
a for-profit corporation? | No |
Does consent expressly permit collection of genetic information? | No |
Does consent expressly permit storage of genetic information? | No |
Does consent prevent dissemination of genetic information? | No |
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? | Yes |
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? | Yes |
How may genetic information associated with the cell line be accessed? | Controlled Access |
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? | No |
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? | Yes |
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? | Yes |
Does consent permit access to medical records of the donor? | Yes |
Please describe how access is provided: | |
Does consent permit access to any other source of information about the clinical treatment or health of the donor? | No |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | No |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | No |
Do you have obligations to third parties in regard to the use of the cell line? | No |
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? | No |
Is there an MTA available for the cell line? | No |
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | CytoTune™-iPS 2.0 Sendai Reprogramming Kit |
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
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Source cell line name |
PBMC Derived from same source line (potentially other lot and donor, see below):
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Source cell type |
An immature, nucleated erythrocyte occupying the stage of erythropoiesis that follows formation of erythroid progenitor cells and precedes formation of reticulocytes.
Synonyms
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Source cell origin |
Blood drawn from a limb.
Synonyms
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Age of donor (at collection) | 20-24 |
Collected in | 2022 |
Passage number reprogrammed | 4 |
Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Is reprogramming vector detectable? |
Yes |
Methods used |
RT-PCR
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Notes on reprogramming vector detection | The absence of the Sendai virus genome and tranfer vector at passage 22 |
Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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Type of used vector free reprogramming factor(s) |
None
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Other |
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Selection criteria for clones | Colonies typical morphology comprised of highly packed cells and well-defined borders with a high nucleus-cytoplasm ratio and prominent nucleoli |
Derived under xeno-free conditions |
Yes |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions
Surface coating | Vitronectin |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
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CO2 Concentration | 5 % |
Medium |
mTeSR™ 1
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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Alkaline Phosphatase |
Yes |
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Marker | Present | Absent |
mCpG | ||
OCT4 |
Differentiation Potency
Microbiology / Virus Screening |
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HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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