iPSC-MARCH-AFC-001

General

Cell Line

hPSCreg name IPi002-A
Cite as:
IPi002-A (RRID:CVCL_D6P8)
Alternative name(s)
iPSC-MARCH-AFC-001
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
Last update 1st March 2024
Notes hiPSC line derived form MARCH amniotic fluid cells Original source of amniotic fluid cells : 10.1136/jmedgenet-2016-104296 Pathological mutation in CEP55 : c.1274C>A, p.Ser425Stop
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Provider

Generator Institut Pasteur (IP)

External Databases

BioSamples SAMEA115370015
Cellosaurus CVCL_D6P8

General Information

* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • MARCH syndrome

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA115370016

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. pfrosk@hsc.mb.ca
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information pfrosk@hsc.mb.ca
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Unknown
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? University of Manitoba - Health Research Ethics Board
Approval number HS16978 (H2013:415)
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
The fluid within the amniotic cavity which surrounds and protects the developing embryo. It is initially produced by the amnion and then later by the lungs and kidneys. The amount at term normally varies from 500 to 2000 ml.
Synonyms
  • Liquor Amnii
  • Amniotic Fluid
  • Waters (Amniotic Fluid)
  • Human Amniotic Fluid
  • FLUID, AMNIOTIC
  • Aqua Amnii
  • AMNIOTIC FLUID
  • Amniotic fluid
  • amniotic fluid
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium mTeSR™ Plus

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal ; 46,XY
Passage number: 10
Karyotyping method: Molecular karyotyping by SNP array
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Other Genotyping (Cell Line)