Generation of two B2M knockout induced pluripotent stem cell lines (DHMi005-A-8 and DHMi005-A-9) using CRISPR/Cas9 technology
Summary
The transplantation of cells into a recipient organism has many hurdles to overcome, including the problem of T-cell-triggered cellular immune defense. Cellular rejection is based on antigen presentation by the MHC-I-complex, which is recognized by cytotoxic T lymphocytes. Elimination of the MHC-I-complex by knocking out the B2M (Beta-2-microglobulin) subunit may be one way of reducing or even completely preventing the initial cellular immune response during transplantation. Using CRISPR/Cas9 we established one heterozygous and one homozygous B2M knockout induced pluripotent stem cell (iPSC) line as a first step towards more effective cell transplantation. Copyright © 2025 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Abdel-Malek K, von Eisenhart-Rothe F, Stiegmann S, Gottmann I, Doppler SA, Schneider S, Preisler H, Lahm H, Dreßen M |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2025 Sep;87:103785 |
| PubMed | 40784045 |
| DOI | 10.1016/j.scr.2025.103785 |