Generation of a human iPSC line (IMEDEAi008-A) derived from natural homozygous CCR5-Δ32 PBMCs enriched in the pro-erythroblast population


A 32 base pair deletion in the C-C chemokine receptor type gene (CCR5-Δ32), the main Human Immunodeficiency Virus (HIV) co-receptor results in a non-functional protein. Individuals homozygous for the CCR5-Δ32 mutation are resistant to HIV infection. Here we report the generation, from pro-erythroblast enriched Peripheral Blood Mononuclear Cells (PBMCs) from a naturally occurring CCR5-Δ32/Δ32 individual, of the fully characterized iPSC line IMEDEAi008-A. The new line has normal karyotype, carry the Δ32 mutation in homozygosity, is free of plasmid integrations, express high levels of pluripotency markers and can differentiate into all three germ layers. Copyright © 2020. Published by Elsevier B.V.

Authors Vallejo-Diez S, Martín-Fernández JM, Sánchez-Gilabert A, Fleischer A, Gayá A, Castresana M, Bachiller D
Journal Stem cell research
Publication Date 2020 Jul 17;47:101918
PubMed 32702648
DOI 10.1016/j.scr.2020.101918

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