WIBR2

General#

Cell Line

hPSCreg Name WIBRe002-A
Alternative name(s)
WIBR2
Cell line type Human embryonic stem cell (hESC)
Last update 16th January 2018
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Provider

Generator Whitehead Institute for Biomedical Research (WIBR)
Owner Whitehead Institute for Biomedical Research (WIBR)
Distributors
Derivation country United States

External Databases

BioSamples SAMEA2660497
Cellosaurus CVCL_9766
NIHhESC NIHhESC-10-0075

General Information

Publications
Projects
Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex female
Ethnicity unknown

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Family history unknown
Is the medical history available upon request? no
Is clinical information available? no

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46XX

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104129950

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2010-06-21
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
Derived under xeno-free conditions?
No
Derivation under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
TrypLE
O2 Concentration 5 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
Differentiation Potency

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)