Generation of heterozygous (MCRIi031-A-1) and homozygous (MCRIi031-A-2) SOX9 knockout human iPSC lines
Summary
The transcription factor SOX9 plays a critical role in several embryonic developmental processes such as gonadogenesis, chrondrogenesis, and cardiac development. We generated heterozygous (MCRIi031-A-1) and homozygous (MCRIi031-A-2) SOX9 knockout induced pluripotent stem cell (iPSC) lines from human fibroblasts using a one-step protocol for CRISPR/Cas9 gene-editing and episomal-based reprogramming. Both iPSC lines exhibit a normal karyotype and morphology, express pluripotency markers, and have the capacity to differentiate into the three embryonic germ layers. These cell lines will allow us to further explore the role of SOX9 in critical developmental processes. Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Pachernegg S, Robevska G, G A Ferreira L, van den Bergen JA, Vlahos K, Howden SE, Sinclair AH, Ayers KL |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Sep;79:103484 |
| PubMed | 38924973 |
| DOI | 10.1016/j.scr.2024.103484 |