Generation of a homozygous CRISPR/Cas9-mediated knockout H9 hESC subline for the CRB1 locus
Mutations in the CRB1 gene reportedly cause early-onset autosomal recessive retinitis pigmentosa (RP), which can result in severe loss of vision at an early age. To investigate the mechanism of CRB1-knockout (CRB1-/-) induced RP, we generated a subline of H9 human embryonic stem cells harboring frame shift mutations in a homozygous state in exon 2 of the CRB1 gene. This subline expressed pluripotent stem cell markers, presented a normal karyotype, and preserved the ability to differentiate into endodermal, mesodermal, and ectodermal lineages. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.
|Authors||Tang X, Chen Z, Tan X, Luo L, Liu X, Gong L, Li DW, Liu Y|
|Journal||Stem cell research|
|Publication Date||2020 Dec;49:102057|