Generation of a homozygous CRISPR/Cas9-mediated knockout H9 hESC subline for the CRB1 locus

Summary

Mutations in the CRB1 gene reportedly cause early-onset autosomal recessive retinitis pigmentosa (RP), which can result in severe loss of vision at an early age. To investigate the mechanism of CRB1-knockout (CRB1-/-) induced RP, we generated a subline of H9 human embryonic stem cells harboring frame shift mutations in a homozygous state in exon 2 of the CRB1 gene. This subline expressed pluripotent stem cell markers, presented a normal karyotype, and preserved the ability to differentiate into endodermal, mesodermal, and ectodermal lineages. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Tang X, Chen Z, Tan X, Luo L, Liu X, Gong L, Li DW, Liu Y
Journal Stem cell research
Publication Date 2020 Oct 16;49:102057
PubMed 33128952
DOI 10.1016/j.scr.2020.102057

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