VUB02
VUBe002-A
General
Cell Line |
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| hPSCreg name | VUBe002-A |
| Cite as: | VUBe002-A (RRID:CVCL_9839) |
| Alternative name(s) |
VUB02
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| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 15th October 2021 |
| User feedback | |
Provider |
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| Generator | Vrije Universiteit Brussel (VUB) |
| Derivation country | Belgium |
External Databases |
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| Cellosaurus | CVCL_9839 |
| Wikidata | Q54993385 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Alternative consent approval number | |
| How may genetic information associated with the cell line be accessed? | |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Federal Commission Medical and Scientific Research on embryos in vitro (Belgium) |
| Approval number | |
| Is there an MTA available for the cell line? | Yes |
hESC Derivation
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
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| ICM morphology |
D
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| Trophectoderm morphology |
g
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| ZP removal technique | Enzymatic |
| Cell isolation | Immunosurgery |
| Cell seeding | Isolated ICM |
Culture Conditions
| Surface coating | MEF | ||||||
| Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
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| O2 Concentration | 20 % | ||||||
| CO2 Concentration | 10 % | ||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| SSEA-1 |
No |
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| DNMT3B |
Yes |
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| GDF3 |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| LIN28 |
Yes |
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| NANOG |
Yes |
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| NPM1 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XY
Passage number: 21
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Other Genotyping (Cell Line) |
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