iEBS

The cell line is not validated yet.

General#

Cell Line

hPSCreg Name MLi002-A
Alternative name(s)
iEBS
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 14th December 2018
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Provider

Generator Faculty of Medicine University of Ljubljana (ML)
Owner Faculty of Medicine University of Ljubljana (ML)
Derivation country Slovenia

External Databases

BioSamples SAMEA5340004

General Information

Is the cell line available in principle?
No

Donor Information#

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
epidermolysis bullosa simplex
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Epidermolysis bullosa simplex
Genetic variants
Keratin 5 (target)
Heterozygous
20128788
Disease associated phenotypes
  • EB Simplex Dowling Meara

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unkown

External Databases (Donor)

BioSamples SAMEA5340319

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
Keratinocyte
Source cell origin
Skin Biopsy
Removal of a portion of skin tissue, for microscopic examination.
Synonyms
  • Biopsy of Skin
  • Skin Biopsy
Passage number reprogrammed 3

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
Yes
Methods used
PCR, RT-PCR, Sequencing
Notes on reprogramming vector detection CytoTune- iPS 2.0 Sendai Reprogramming kit from Invitrogen / After ten passages, the elimination of the SeV vectors was confirmed in the MLi002-A cell line by RT-PCR using specific primers
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells Human fibroblast feeders
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
O2 Concentration 5 %
CO2 Concentration 5 %
Medium mTeSR™ 2
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Alkaline Phosphatase
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46 XX
Karyotyping method: Array CGH

Other Genotyping (Cell Line)