Generation of a human induced pluripotent stem cell line (SIAISi010-A) from a 31-year-old healthy donor with Chinese Han genetic background
Summary
A healthy 31-year-old Chinese Han female donated peripheral blood mononuclear cells (PBMC). Her PBMCs were reprogrammed with human OKSM (OCT3/4, KLF4 SOX2, and c-MYC) transcription factors by the non-integrating episomal vector system. Immunocytochemistry for pluripotency markers confirmed the pluripotency of transgene-free iPSCs. Their ability to differentiate spontaneously three germ layers in vitro is also confirmed. The iPSC line displayed a normal karyotype. This model can be used as a control in pathological mechanism studies. Copyright © 2021. Published by Elsevier B.V.
| Authors | Dai Q, Di W, Zhang Y, Gu S, Zhao J, Zhang B, Wang Y |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 May;53:102314 |
| PubMed | 33799282 |
| DOI | 10.1016/j.scr.2021.102314 |