Lp(a) Cl23

General

Cell Line

hPSCreg name ITXi012-A
Cite as:
ITXi012-A (RRID:CVCL_D0GM)
Alternative name(s)
Lp(a) Cl23
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 5th April 2023
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Provider

Generator l’institut du thorax (ITX)
Owner l’institut du thorax (ITX)
Distributors
Derivation country France

External Databases

BioSamples SAMEA112371431
Cellosaurus CVCL_D0GM
Wikidata Q123032756

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • Hyperlipoproteinemia
Disease associated phenotypes
  • Hyperlipoproteinemia

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

External Databases (Donor)

BioSamples SAMEA112366216

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. Bertand Cariou (bertrand.cariou@univ-nantes.fr)
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Authorization number from the French Ministry of Health
Approval number DC-2011-1399
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell line name Lp(a) Cl23
Source cell type
An excreta material which is composed primarily of urine, a liquid excreta containing water and waste products made by the kidneys, stored in the bladder, and leaves the body through the urethra.; ENVO bodily fluid classes differ from UBERON's treatment of bodily fluids. UBERON refers to the substance itself ("S"). We assert that UBERON's classes are the primary compositional component of the terms in ENVO ("S material"). Use of the ENVO terms is typically recommended when you wish to indicate that there may be other materials intermixed with S.
Source cell origin
The fluid that is excreted by the kidneys. It is stored in the bladder and discharged through the urethra.
Synonyms
  • Urine
  • Urinary System, Urine
  • urine
  • URINE
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Episomal
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Yes
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
O2 Concentration 4 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemMACS iPS-Brew medium
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Unknown

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-60
Yes
POU5F1 (OCT-4)
Yes
NANOG
Yes
SOX2
Yes
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
FOXA2
Yes
Morphology
Endo_Meso_Ecto relative expression.pdf
ITXi012-A Differentiation capacity Endo, Meso et Ecto
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
HAND1
Yes
Morphology
Endo_Meso_Ecto relative expression.pdf
ITXi012-A Differentiation capacity Endo, Meso et Ecto
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
PAX6
Yes
Morphology
Endo_Meso_Ecto relative expression.pdf
ITXi012-A Differentiation capacity Endo, Meso et Ecto

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Unknown

Other Genotyping (Cell Line)