Generation of a CAG-EGFP tagged cell line (KSCBi017-A-2) from human induced pluripotent stem cells using CRISPR/Cas9
Summary
Human induced pluripotent stem cells (hiPSCs) have potential use in regerenrative medicine for disease modeling and drug screening studies. The AAVS1 locus has been validated as a stable transgene expression and safe genomic location. Therefore, we inserted the enhanced green fluorescent protein (EGFP) gene into the AAVS1 locus of hiPSCs, using CRISPR/Cas9 genome editing. The results showed that the hiPSCs stably expressed EGFP in pluripotency and differentiated into three germ lineages. Our results strongly indicate that the EGFP-tagged cell line has potential for use in in vivo and in vitro experiments for monitoring cell location and type. Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Han HW, Im YS, Kim YO, Kwak S, Cho SJ |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Mar;75:103303 |
| PubMed | 38211411 |
| DOI | 10.1016/j.scr.2024.103303 |