ES-76
EVMSe001-A
General
Cell Line |
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| hPSCreg name | EVMSe001-A |
| Cite as: | EVMSe001-A (RRID:CVCL_C079) |
| Alternative name(s) |
ES-76
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
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| Generator | Eastern Virginia Medical School (EVMS) |
| Derivation country | United States |
External Databases |
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| Cellosaurus | CVCL_C079 |
| Wikidata | Q54832971 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent |
hESC Derivation
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
| PGD Embryo? |
No |
| Cell seeding | Isolated ICM |
Culture Conditions
| Surface coating | PMEF | |||||||||
| Feeder cells |
Primary Mouse Embryonic Fibroblasts (PMEF) Cellfinder Ont Id: EFO_0004040 |
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| Medium |
Other medium:
Base medium: DMEM
Main protein source: Fetal bovine serum Serum concentration: 20 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| SSEA-1 |
No |
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| SSEA-4 |
Yes |
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Genotyping
Karyotyping (Cell Line) |
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Other Genotyping (Cell Line) |
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