ES-76

General#

Cell Line

hPSCreg Name EVMSe001-A
Alternative name(s)
ES-76
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator Eastern Virginia Medical School (EVMS)
Derivation country United States

External Databases

Cellosaurus CVCL_C079

General Information

Publications
Is the cell line available in principle?
No

Donor Information#

General Donor Information

Phenotype and Disease related information (Donor)

Diseases

Ethics#

We are currently revising this section.

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
Cell seeding Isolated ICM

Culture Conditions#

Surface coating PMEF
Feeder cells Primary Mouse Embryonic Fibroblasts (PMEF)
Cellfinder Ont Id: EFO_0004040
Medium Other medium:
Base medium: DMEM
Main protein source: Fetal bovine serum
Serum concentration: 20 %
Supplements
L-Glutamine
LIF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SSEA-1
No
SSEA-4
Yes

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)