NCL-6

General

Cell Line

hPSCreg name UNEWe006-A
Cite as:
UNEWe006-A (RRID:CVCL_A007)
Alternative name(s)
NCL-6
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 10th April 2017
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Provider

Generator University of Newcastle (UNEW)
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_A007
Wikidata Q54908234

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent
Name of accrediting authority involved?
Approval number

hESC Derivation

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Chemical
Cell isolation Immunosurgery
Cell seeding Embryo

Culture Conditions

Surface coating Gelatin
Feeder cells Human fetal lung cell
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
Medium conditioned by hES-NCL1 cells %
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SSEA-4
Yes
Alkaline Phosphatase
Yes
ZFP42 (REX-1)
Yes
POU5F1 (OCT-4)
Yes
NANOG
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46 XX
Passage number: 20

Other Genotyping (Cell Line)