Shef-6

General#

Cell Line

hPSCreg Name UOSe006-A
Alternative name(s)
Shef-6
Cell line type Human embryonic stem cell (hESC)
Last update 8th September 2017

Provider

Generator University of Sheffield (UOS)
Owner University of Sheffield (UOS)
Distributors National Institute for Biological Standards and Control - UK Stem Cell Bank (USCB)
University of Sheffield (UOS)
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_9793

General Information

Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex other

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Ethics#

We are currently revising the public data view for cell lines registered in hPSCreg. This section will be visible again in the next weeks.

hESC Derivation#

Date of derivation 2005-02-10
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique 0
Cell isolation 0
Cell seeding 0
Derived under xeno-free conditions?
No
Derivation under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Non coated
Feeder cells mouse feeders
Passage method Mechanically
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: KO-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Protocol or reference
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Protocol or reference
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Protocol or reference

Microbiology / Virus Screening

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Genetic Modifications (other than for reprogramming)