VCCRIi002-A

069

General

Cell Line

hPSCreg name VCCRIi002-A
Cite as:
VCCRIi002-A (RRID:CVCL_D0PE)
Alternative name(s)
069
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
Last update 12th December 2023
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Provider

Generator Victor Chang Cardiac Research Institute (VCCRI)
Owner Victor Chang Cardiac Research Institute (VCCRI)
Distributors
Derivation country Australia

External Databases

BioSamples SAMEA114465051
Cellosaurus CVCL_D0PE
Wikidata Q123033768

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:

Restrictions subject to Ethics

Donor Information

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is affected.
Stage
Recurrent SCAD (3 SCAD Events)
Synonyms
  • Spontaneous Coronary Artery Dissection
  • SCAD
Synonyms
  • FMDA
  • Fibromuscular hyperplasia of arteries NOS (disorder)
  • Fibromuscular hyperplasia of artery (disorder)
  • fibromuscular dysplasia of arteries
  • Fibromuscular Dysplasia
  • fibromuscular dysplasia
  • Fibromuscular hyperplasia of artery [Ambiguous]
  • Fibromuscular hyperplasia of artery
  • Fibromuscular dysplasia (morphologic abnormality)
show more synonyms
Family history Sibling with SCAD
Is the medical history available upon request? Available in accordance with ethics
Is clinical information available? Available in accordance with ethics

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

Donor Relations

All cell lines of this donor's relatives
Has sister:

External Databases (Donor)

BioSamples SAMEA114465044

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? No
a non-profit company? No
a for-profit corporation? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? St Vincent's Hospital Human Research Ethics Committee
Approval number HREC/16/SVH/338
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC
Source cell origin
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
  • Reticuloendothelial System, Blood
  • Peripheral Blood
  • peripheral blood
  • blood
  • Blood
  • Whole Blood
  • BLOOD
  • portion of blood
  • vertebrate blood
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 18 %
CO2 Concentration 5 %
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SSEA-4
Yes
Morphology pictures
Brightfield Example of Morphology of Line 069
Self-renewal
Positive
Endoderm
Negative
Mesoderm
Negative
Ectoderm score
Negative
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Scorecard
Morphology
Sox17 expression (green) from endodermal differentiation 69
Supplementary Figure 3 Scorecard.pdf
Scorecard Line 69 Trilineage
Protocol or reference
Methods for hPSCreg.JPG
Methods from hPSCreg
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Scorecard
Marker Expressed
Brachyury
Yes
Morphology
Mesodermal Progenitor Brachyury (green) DAPI (blue)
Protocol or reference
Methods for hPSCreg.JPG
Methods from hPSCreg
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Scorecard
Marker Expressed
PAX6
Yes
Morphology
Ectodermal Expression of Pax6 (green) and DAPI (blue) in Neural Rosettes
Protocol or reference
Methods for hPSCreg.JPG
Methods from hPSCreg

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal
Passage number: 20
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes