GZHMCe004-A

FY-hES-11

General

Cell Line

hPSCreg name GZHMCe004-A
Cite as:
GZHMCe004-A (RRID:CVCL_9930)
Alternative name(s)
FY-hES-11
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 1st September 2022
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Provider

Generator The Third Affiliated Hospital of Guangzhou Medical University (GZHMC)
Derivation country China

External Databases

Cellosaurus CVCL_9930
Wikidata Q54835296

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?

hESC Derivation

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Enzymatic
Cell isolation Mechanical
Cell seeding Isolated ICM

Culture Conditions

Surface coating MEF
Feeder cells mouse embryonic fibroblast cells
Cellfinder Ont Id: EFO_0004040
Passage method Mechanically
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Supplements
L-Glutamine
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-1
No
ZFP42 (REX-1)
Yes
LEFTY1
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
TDGF1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 25

Other Genotyping (Cell Line)