BIHi261-A-1

The cell line is not validated yet.

General

Cell Line

hPSCreg name BIHi261-A-1
Cite as:
BIHi261-A-1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 22nd May 2025
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Provider

Generator Berlin Institute of Health (BIH)

External Databases

BioSamples SAMEA118348716

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • POMC deficiency
Genetic variants
POMC (target)
2p23.3
NM_001319205.2:c.(252G>A)
NP_001306134.1:p.(W84*)
Homozygous

External Databases (Donor)

BioSamples SAMEA118245864

Ethics

Also have a look at the ethics information for the parental line BIHi261-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line BIHi261-A.

Reprogramming method

Vector type Non-integrating
Vector RNA
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 5 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • Obesity
  • Obesity, unspecified
  • obesity
Genetic modifications
Isogenic modification
2p23.3
NC_000002.12(NM_000939.4):c.[246C>T;251A>G]
NM_000939.4(NP_000930.1):p.*84W
Homozygous
Repaired