The cell line is not validated yet.
BIHi261-A-1
General
Cell Line |
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hPSCreg name | BIHi261-A-1 |
Cite as: | BIHi261-A-1 |
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines | No similar lines found. |
Last update | 22nd May 2025 |
User feedback | |
Provider |
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Generator | Berlin Institute of Health (BIH) |
External Databases |
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BioSamples | SAMEA118348716 |
General Information |
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* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Subclone of |
Donor Information
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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External Databases (Donor) |
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BioSamples | SAMEA118245864 |
Ethics
Also have a look at the ethics information for the parental line
BIHi261-A
.
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
BIHi261-A.
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Reprogramming method |
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Vector type | Non-integrating |
Vector | RNA |
Genes | |
Is reprogramming vector detectable? |
No |
Methods used |
PCR
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Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
Unknown |
Derived under GMP? |
Unknown |
Available as clinical grade? |
Unknown |
Culture Conditions
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
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O2 Concentration | 5 % |
CO2 Concentration | 5 % |
Medium |
Essential 8™
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46, XY
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Other Genotyping (Cell Line) |
Genetic Modification
Disease/phenotype related modifications |
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