General

Cell Line
hPSCreg name	BIHi261-A-1
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	BIHi261-A-1
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	No similar lines found.
Last update	22nd May 2025
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Provider
Generator	Berlin Institute of Health (BIH)
External Databases
BioSamples	SAMEA118348716
General Information
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Clinical use: not allowed Commercial use: not allowed
Subclone of	BIHi261-A

Donor Information

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Obesity due to pro-opiomelanocortin deficiency The donor is a carrier of a disease-associated mutation and affected. Synonyms POMC deficiency Genetic variants POMC (target) Chromosome location 2p23.3 Nucleotide sequence variant in HGVS format NM_001319205.2:c.(252G>A) Protein sequence variant in HGVS format NP_001306134.1:p.(W84*) Is the variant homozygous or heterozygous? Homozygous
External Databases (Donor)
BioSamples	SAMEA118245864

Ethics

Also have a look at the ethics information for the parental line BIHi261-A .

For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
The source cell information can be found in the parental cell line BIHi261-A.
Reprogramming method
Vector type	Non-integrating
Vector	RNA
Genes	SOX2 KLF4 MYC OCT4 GLS1
Is reprogramming vector detectable?	No
Methods used	PCR
Vector free reprogramming
Other
Derived under xeno-free conditions	Unknown
Derived under GMP?	Unknown
Available as clinical grade?	Unknown

Culture Conditions

Surface coating	Matrigel/Geltrex
Feeder cells	No
Passage method	Enzyme-free cell dissociation EDTA
O2 Concentration	5 %
CO2 Concentration	5 %
Medium	Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?	No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?	No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?	No

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
NANOG	Yes
POU5F1 (OCT-4)	Yes
SSEA-4	Yes
TRA 1-60	Yes

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vitro directed differentiation

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vitro directed differentiation

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

In vitro directed differentiation

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46, XY
Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications

Obesity Synonyms Obesity Obesity, unspecified obesity Genetic modifications Type of modification Isogenic modification Chromosome location 2p23.3 Nucleotide sequence variant in HGVS format NC_000002.12(NM_000939.4):c.[246C>T;251A>G] Protein sequence variant in HGVS format NM_000939.4(NP_000930.1):p.*84W Is the modification homozygous or heterozygous? Homozygous How has the target locus been modified? Repaired