General#

Cell Line

hPSCreg Name UBi001-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 30th October 2019
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Provider

Generator University of Barcelona (UB)

External Databases

BioSamples SAMEA6298140

General Information

* Is the cell line readily obtainable for third parties?
No
Subclones

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Sanfilippo syndrome type C
The donor is not a carrier of a disease-associated mutation and not affected.
Synonyms
  • Mucopolysaccharidosis type 3C
  • MPS3C
  • HGSNAT deficiency
  • Heparan-alpha-glucosaminide N-acetyltransferase deficiency
  • MPSIIIC
  • Mucopolysaccharidosis type IIIC
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: G-Banding

External Databases (Donor)

BioSamples SAMEA6298140

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: The WT fibroblasts were purchased from a private company
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? No
If you do not hold the SIGNED Donor Consent Form, do you know who does? No
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Bioethical Comission of the University of Barcelona (October 20, 2016)
Approval number IRB00003099
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Bioethical Comission of the University of Barcelona (October 20, 2016)
Approval number IRB00003099
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
Fibroblasts

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
No
Reprogramming vectors silenced?
Yes
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Medium mTeSR™ 1

Characterisation#

Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
alpha Smooth Muscle Actin
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
beta3 tubulin
Unknown

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY

Other Genotyping (Cell Line)