UKERi010-A-2

UKERi7MN-010-2

General

iPSC Line

hPSCreg name UKERi010-A-2
Cite as:
UKERi010-A-2 (RRID:CVCL_C1JT)
Alternative name(s)
UKERi7MN-010-2
iPSC line type Human induced pluripotent stem cell (hiPSC)
Similar iPSC lines
UKERi010-A
(UKERi7MN-S1-010)
UKERi010-A-1
(UKERi7MN-010-1)
Last update 8th July 2022
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Provider

Generator Universitätsklinikum Erlangen (UKER)

External iPSC Databases

BioSamples SAMEA110290101
Cellosaurus CVCL_C1JT
Wikidata Q114313173

General iPSC Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: not allowed
Subclone of

iPSC Genetic Modification

Genetic modifications not related to a disease
Gene knock-out
4p22
Heterozygous gene-Knockout of alpha-synuclein; SNCA(+/-)
CRISPR-associated (CRISPR/Cas) System

Donor Information

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46XX
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA110284857

Ethics

Also have a look at the ethics information for the parental line UKERi010-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

iPSC Derivation

General

The source cell information can be found in the parental cell line UKERi010-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

iPSC Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
O2 Concentration 0 %
CO2 Concentration 5 %
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at thaw previously with this iPSC line?
Yes

iPSC Characterisation

No characterisation data could be found for this subclone. Please open parental cell line UKERi010-A .

Genotyping

Karyotyping (iPSC Line)

Has the iPSC line karyotype been analysed?
Yes
46XX
Passage number: 26
Karyotyping method: G-Banding

Other Genotyping (iPSC Line)