UKERi7MN-010-2
UKERi010-A-2
General
Cell Line |
|
| hPSCreg name | UKERi010-A-2 |
| Cite as: | UKERi010-A-2 (RRID:CVCL_C1JT) |
| Alternative name(s) |
UKERi7MN-010-2
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
UKERi010-A (UKERi7MN-S1-010) UKERi010-A-1 (UKERi7MN-010-1) |
| Last update | 8th July 2022 |
| User feedback | |
Provider |
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| Generator | Universitätsklinikum Erlangen (UKER) |
External Databases |
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| BioSamples | SAMEA110290101 |
| Cellosaurus | CVCL_C1JT |
| Wikidata | Q114313173 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
46XX
Karyotyping method:
G-Banding
|
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA110284857 |
Ethics
Also have a look at the ethics information for the parental line
UKERi010-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
UKERi010-A.
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Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
Vector free reprogramming |
|
Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
|
| O2 Concentration | 0 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ Plus
|
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
No characterisation data could be found for this subclone. Please open parental cell line UKERi010-A .Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 26
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Genetic modifications not related to a disease |
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